To evaluate oral stimulation caused by dietary oil, the licking test is useful as it evaluates the reaction to a sample solution within 60 s. In addition to dietary oil, mice prefer low concentrations of FAs solutions. Considering the finding that the lingual lipase digested the triacylglycerol (Kawai and Fushiki, 2003) and released small amounts of FA on the tongue, it is reasonable to conclude that mice prefer low concentrations of FA. However, while creating energy for themselves, the microbes

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